Fig. 3.

Effect of iron deprivation on mycobacterium biofilm. Iron deprivation (2,2 BP (35 μg/ml) and siderophore mutant Δ011-14) showing inhibited biofilm formation of Mycobacterium smegmatis in presence of three known anti-TB drugs, i.e. INH, EMB and RIF (1 μg/ml, 0.0625 μg/ml, 0.0625 μg/ml). (a) Crystal violet staining showing the inhibited biofilm formation under iron deprivation (2,2 BP (35 μg/ml) and siderophore mutant Δ011-14). (b) Biofilm inhibition under iron deprivation (2,2 BP (35 μg/ml) and siderophore mutant (Δ011-14) in presence of three known anti-TB drugs, i.e. INH, EMB and RIF (1 μg/ml, 0.0625 μg/ml, 0.0625 μg/ml) depicted as reduction in growth percent quantified by using crystal violet dye as bar graph. Mean of OD₆₀₀ ± SD of three independent sets of experiments are depicted on Y-axis and * depicts p value <0.05.