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Schematic map of primer annealing sites for junction PCR (J1, J2, J3, J4 and J5); mapping PCR (M1, M2, M3, M4, M5 and M6); and crossing PCR assays. All primers were designed based upon pKP048 (GenBank accession no. FJ628167.2) sequence data, with the exception of the primers corresponding to J4 and J5, which were designed based upon pKPHS2 (GenBank accession no. cp003224.1).
