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. 2000 Jan;182(2):295–302. doi: 10.1128/jb.182.2.295-302.2000

FIG. 3.

FIG. 3

SDS-PAGE analysis of in vivo expression of the lipase and the activator. (A) Expression of act encoded by E. coli BL21(DE3)/LysS bearing pRSET-Act. Lane M, marker proteins; lane 1, whole-cell proteins of E. coli BL21(DE3)/LysS bearing pRSET-Act before induction by IPTG; lane 2, whole-cell proteins of E. coli BL21(DE3)/LysS bearing pRSET-Act after induction by IPTG; lane 3, cell lysate of whole-cell proteins of lane 2; lane 4, activator partially purified by 20% saturated (NH4)2SO4 precipitation. (B) Expression of rlip by E. coli BL21(DE3)/LysS bearing pRSET-rLip. Lane M, marker proteins; lane 1, whole-cell proteins of E. coli BL21(DE3)/LysS; lane 2, whole-cell proteins of E. coli BL21(DE3)/LysS bearing pRSET-rLip induced by IPTG; lane 3, soluble proteins in whole-cell proteins of lane 2; lane 4, insoluble proteins in whole-cell proteins of lane 2.