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. 2015 Dec 31;20(2):160–165. doi: 10.1016/j.bjid.2015.11.002

Fig. 2.

Fig. 2

The role of M. tuberculosis pili (MTP) in epithelial cell cytokine production. A549 epithelial cells were exposed to the wild-type, MTP-deficient mutant, and MTP-overexpressing complemented strains for 4 h. Extracellular bacteria were removed by washing, fresh media added, and cell culture supernatants at 24, 48, and 72 h post-infection were assayed for levels of the cytokines IL-1β (A), IL-4 (B), IL-6 (C), IL-8 (D), G-CSF (E), IFN-γ (F), MCP-1 (G), and TNF-α (H), using a Bio-Plex assay (Bio-Rad). Error bars are ± 1 × SEM.