Figure 4. IL-23-induced skin inflammation is not associated with expansion of dermal γδ T cells.

Expression profiling of ear tissue collected from WT and TCR^δ−/− mice post GFP and/or IL-23 gene transfer, showing (A) Hierarchical clustering heatmap plot of top 30 differentially expressed genes (DEG) and (B) GO enrichment analysis for the upregulated genes post GFP and/or IL-23 gene transfer. (C) TCR repertoire analysis, depict in pie charts the distribution of TRD CDR3 in each treatment group cluster, different colors represent different TRD CDR3 sequences. The relative abundance of each TRD CDR3 sequence within each group is shown as percentage (%) beside the charts. (Representative data of at three independent experiments 3 mice per group). (D) Representative flow cytometry dot plots pre-gated on live lymphocytes and (E) bar graphs showing the gating strategy and percentage of CD3⁺γδTCR⁺GM-CSF⁺IL-17A⁺ cells in the skin of WT mice after 11 days post IL-23 and/or GFP gene transfer. All data are shown as mean ± SEM as determined by unpaired student’s t-test. *P<0.05; ** P<0.01; *** P<0.001, ns = not significant. (F) GM-CSF mRNA expression in ears isolated from WT and/or TCR^δ−/− mice 11 days post GFP and/or IL-23 MC gene transfer.