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. 2022 Aug 30;8:100. doi: 10.1038/s41523-022-00472-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Immunohistochemical staining for GPER with different intensity. The staining is graded as negative (−), weak (+), moderate (++), and strong (+++). Scale bar, 100 μm. b Scatter plots show that the IHC score is linearly correlated with the log2 expression value of GPER in the results of Pearson correlation coefficient calculation, which is statistically significant. Pearson correlation coefficient R² and p-value are given in the scatter plot. c Image showing IHC staining of GPER is only observed in cytoplasmic. Scale bar, 50 μm. d Representative image of weak GPER staining in the core area and strong GPER staining in the corresponding margin. Scale bar, 100 μm.