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. 2022 Aug 30;12:14747. doi: 10.1038/s41598-022-18413-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of multiple markers on NK cells among the study groups. The analysis of CD8 and KIR3DL1/S1 expression was quantified on total NK cells comprising CD56^brightCD16⁻, CD56^dimCD16^bright, and CD56^−veCD16^bright, whereas the expression of NKG2C, CD2, CD7, CD57, and HLA-DR were determined from NK cells comprising the CD56^bright and CD56^dim subsets. A total of 15 subjects were evaluated for HIV and 15 for HC subjects for CD8 and KIR markers; 13 (HIV) and 14 (HC) subjects for NKG2C, CD2, CD7; and 13 (HIV) and 16 (HC) subjects for CD57 and HLA-DR marker expression. Abbreviations on X-axis refer to the different NK cell markers. Mann–Whitney tests were performed with a p-value < 0.05 considered statistically significant. The median and 25th and 75th percentile are indicated on each plot.