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. 2022 Aug 30;12:14763. doi: 10.1038/s41598-022-19166-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of sleep fragmentation, RAS manipulation, and their interaction on Il-1β, TGFβ, and TNFα gene expression in peripheral tissues, epididymal white adipose tissue (EWAT; A, B, C), liver (D, E, F), and spleen (G, H, I). Samples sizes are (A, B) NSF-Con n = 11, NSF-Ang n = 10, NSF-Cap n = 9, SF-Con n = 10, SF-Ang n = 9, and SF-Cap n = 9, (C) NSF-Con n = 11, NSF-Ang n = 10, NSF-Cap n = 10, SF-Con n = 10, SF-Ang n = 9, and SF-Cap n = 9, (D) NSF-Con n = 10, NSF-Ang n = 9, NSF-Cap n = 10, SF-Con n = 10, SF-Ang n = 8, and SF-Cap n = 10, (E) NSF-Con n = 10, NSF-Ang n = 9, NSF-Cap n = 10, SF-Con n = 9, SF-Ang n = 9, and SF-Cap n = 9, (F) NSF-Con n = 11, NSF-Ang n = 9, NSF-Cap n = 10, SF-Con n = 10, SF-Ang n = 9, and SF-Cap n = 10, (G) NSF-Con n = 10, NSF-Ang n = 9, NSF-Cap n = 9, SF-Con n = 10, SF-Ang n = 9, and SF-Cap n = 9, (H) NSF-Con n = 10, NSF-Ang n = 9, NSF-Cap n = 8, SF-Con n = 10, SF-Ang n = 8, and SF-Cap n = 9, (I) NSF-Con n = 10, NSF-Ang n = 9, NSF-Cap n = 9, SF-Con n = 10, SF-Ang n = 8, and SF-Cap n = 9. All data were analyzed using a two-way ANOVA and Tukey’s HSD post hoc tests. Data shown as means ± 1 SE for each group and differing lowercase letters denotes p < 0.05.