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. 2022 Aug 30;12:14749. doi: 10.1038/s41598-022-18999-8

Table 4.

Primers designed for the RT-PCR gene expression analysis of G6PD, Ntan1 and Ntan1 transcripts.

Gene/transcript Primers (5′→ 3′) Amplicon size (bp)
G6PD

F: AGTCGCCTACAATGGTCTGC

R:GTTCGAATCGTTGCTAACGG

gDNA: 1106

cDNA: 959

Ntan1

F: GGTGTGCTGCTGCAGGATGACT

R: CGTAGTTAAAGGGTCCAATGC

gDNA: 901

cDNA: 685

Ntan1-RA

F: GTGCACGAATCGCTGAACT

R: CGTTCCCAGTTCAGTTCAGATCCAT

cDNA:1136
Ntan1-RB

F: GTCTCTCTTTCCGGTTTGCGTC

R: CGTTCCCAGTTCAGTTCAGATCCAT

cDNA: 1336
Ntan1-RC

F: GTGCACGAATCGCTGAACT

R: CGTTCCCAGTTCAGTTCAGATCCAT

cDNA: 1405
Ntan1-RD

F: GTCTCTCTTTCCGGTTTGCGTC

R: CGTTCCCAGTTCAGTTCAGATCCAT

cDNA: 1601

The corresponding amplicon size for each primer pair is also indicated.