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. 2022 Aug 30;13:5114. doi: 10.1038/s41467-022-32791-2

Fig. 5. CPE motif in the 3’UTR regulate the oocyte secreted proteins.

Fig. 5

a The translational expressions of OOSP gene family in human and mouse GV and MII oocytes. The CPE and PAS elements and positions at the 3’UTRs are indicated on the top of each mouse and human OOSP gene. are indicated on the right of mouse and human OOSP genes. Data from 2 biological replicates of 10 human oocytes and 3 biological replicates of 10 mouse oocytes. Error bar denotes SD. b Translational expressions of CPEB2 and CPEB4 n = 5 (including 10 and single oocyte data). Data are presented as mean values ± SD. c 3’-UTR Luciferase reporter assays of CPEB4 transfected with wild-type or the two mutated CPEB4 elements at the 3’-UTR of OOSP2. n = 4, (four independent biological replicates per 293FT cell populations, ∼25,000 cells in each sample, Unpaired two-sided t-test). Data are presented as mean values ± SD. d Translation expression levels of human MII enriched secreted proteins at GV and MII stages. Data from two biological replicates of 10 human oocytes. Error bar denotes SD. e 3’UTRs of the MII highly translated and secreted proteins showing the number and approximate location of CPE and PAS motifs.