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. 2022 Aug 30;13:5114. doi: 10.1038/s41467-022-32791-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Pseudotime analysis showing the developmental stages of GV control oocytes, hRec-OOSP2 treated oocyte, ab-OOSP2 treated oocytes, MII oocytes, and morula embryos. b Volcano plot showing transcriptome DEGs of GV control oocytes versus hRec-OOSP2 treated oocyte (p-value, wald test) c Volcano plot showing translatome DEGs of GV control oocytes versus hRec-OOSP2 treated oocyte (p-value, wald test). d Volcano plot showing the TE changes after treated with hRec-OOSP2. TE of each gene is depicted as log2TE of control and hRec-OOSP2 treated oocytes. Red dot indicates upregulated genes in hRec-OOSP2 treated oocytes. Blue dot indicates GV enriched genes. The darker the color, the higher the TE. e Putative RBPs (top10 fold change) identified by 3’UTR motifs of TE > 4 genes after treated with hRec-OOSP2 (p-value, one-sided t-test). f Representative GO terms of TE > 4 genes after treated with hRec-OOSP2 (p-value, hypergeometric test). g, h T&T expression heatmap of the OOSP2 upregulated translational genes involved in small GTPase signaling pathway and organelle localization.