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. 2022 Aug 30;12:14730. doi: 10.1038/s41598-022-19172-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Aberrant expression of miR-133a-3p strongly inhibits endothelial cell migration. (A) Representative images of wound-healing migration assays performed with HUVEC transfected with microRNA mimics for miR-133a-3p, miR-133a-5p or a negative control (miR-NC). Images were taken at time zero (0 h) and after incubation for 24 h (24 h). The migrated area was calculated using ImageJ software (https://imagej.nih.gov/ij/) by subtracting the value of the non-migrated area from the wound area at time zero and expressing this as a percentage of the total area at time zero. Scale bars, 1000 μm. Data are shown as mean ± SE, n = 6. Data were analysed for statistical differences by one-way ANOVA with post hoc Tukey’s comparison test. ns = non-significant; ***, P ≤ 0.001; ****, P ≤ 0.0001. (B,C) TaqMan gene expression assay analysis of RNA levels for MSN and PLAUR in HUVEC transfected with microRNA mimics as above. Data are shown as mean ± SE, n = 9. *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.0001 one-way ANOVA with post hoc Tukey’s comparison test for the indicated groups.