Fig. 4. Ablation of biliary NIK ameliorates DDC-induced liver injury and inflammation.
A–C NIKΔK19 and NIKf/f male mice were fed a DDC diet for 4 weeks. A Plasma ALT, ALP and total bilirubin levels. NIKf/f: n = 8 mice, NIKΔK19: n = 9 mice. B, C Liver sections were stained with H&E or antibodies to CD11b, and Gr-1. B Representative images. Scale bar: 200 μm. C CD11b (n = 3 mice per group) and Gr-1 cells (n = 3 mice per group) were counted and normalized to total liver cell number (n = 3–4 mice per group). D, E NIKΔK19 and NIKf/f male mice were fed an ANIT diet for 3 weeks. D Representative H&E staining of liver sections. Scale bar: 200 μm. E Liver sections were stained with anti-CD11b antibody. CD11b cells were counted and normalized to total liver cells. NIKf/f: n = 7 mice, NIKΔK19: n = 6 mice. Data are presented as mean ± SEM. *p < 0.05, 2-way ANOVA (A) and 2-tailed student’s t-test (C, E). Source data are provided as a Source Data file.