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. 2022 May 30;127(5):811–823. doi: 10.1038/s41416-022-01834-2

Fig. 2. Upregulation of PFKFB3 contributes to the resistance of gastric cancer cells to trastuzumab.

Fig. 2

a Representative IHC images of PFKFB3 in tumour from trastuzumab-resistant patients and PDX models compared with the control group, respectively. Scale bar, 100 μm (×40). b Relative glucose consumption and lactate release in cultural supernatant of MKN45-TRs and NCI-N87-TRs (NC vs. siPFKFB3) after culturing under normoxia (20% oxygen) or hypoxia (1% oxygen) for 48 h. c Western blot analysis for glycolytic genes in siPFKFB3 transfected TRs relative to the negative control (NC). d The MKN45-TRs and NCI-N87-TRs (NC vs. siPFKFB3) were cultured in normal or low glucose conditions (0.5 mM glucose) for a short period (48 h), followed by MTT assays. e Stable NCI-N87-WT cells transfected with either shNC or shPFKFB3 were subcutaneously inoculated in athymic nude mice (n = 3/group). Macroscopic appearance of subcutaneous tumour of mice (Upper). Tumour volume at sacrifice (lower left). Tumour weight at sacrifice (lower right). Each experiment was performed at least triplicately and results are presented as mean ± SEM. Student’s t test, one-way ANOVA or two-way ANOVA was used to analyse the data (*P < 0.05, **P < 0.01, ***P < 0.001).