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. 2022 May 30;127(5):811–823. doi: 10.1038/s41416-022-01834-2

Fig. 5. PFKFB3 promotes the transcription and secretion of CXCL8 by phosphorylating the S1151 site of ERBB2.

Fig. 5

a High-throughput phosphorylation profiling with 3057 site-specific antibodies from 10351 phosphorylation sites. 352 unique sites were up-/downregulated significantly (107 sites up, 145 sites down) according to P value < 0.05 (fold change >1.5, CV < .1). b The gene ontology (GO) enrichment analyses the function of differentially expressed proteins and presented as a network. c The proteomic analysis identified a spectrum of ERBB2 proteins to communicate with PFKFB3 and CXCL8 protein and presented as a network. d Representative MS/MS fragmentation spectra of phosphopeptide depicting ERBB2 Ser1151 phosphorylation enriched from PFKFB3-overexpressed cells. e Western blot was used to evaluate the phosphorylation level of ERBB2 in S1151 site after PFKFB3 transfected. f Endogenous interaction between PFKFB3 with p-ERBB2 S1151 in TRs by Co-IP assays. g The location of PFKFB3 and p-ERBB2 S1151 in PFKFB3-overexpressed cells was assessed by IF staining.