TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description or construction | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α F′ | φ80ΔlacZΔM15 endA1 relA1, hsdR17 (r−, m+) supE44 thi-1 λ gyrA relA1 F− Δ(lacZYA-argF)U169 | 16 |
| S17-1 | E. coli 294::RP4-2 (Tc::Mu)(Km::Tn7) | 43 |
| YB1329 | S17-1 λpir pUT::miniTn5lacZ2 | 9; Alley, unpublished results |
| C. crescentus | ||
| NA1000 | Previously called CB15N, a synchronizable derivative of CB15, does not synthesize a holdfast | 12 |
| AE1045 | NA1000 flaN::Tn5lacZ Skl | Alley, unpublished results |
| YB720 | NA1000 phoBΩ12 | This work |
| YB1630 | NA1000 pstS::miniTn5lacZ1100 | 42 |
| YB767 | NA1000 pstS::miniTn5lacZ1100, transduction of Kanr from YB1630 | This work |
| YB770 | NA1000 phoBΩ12 pstS::miniTn5lacZ1100, transduction of Kanr from YB1630 into YB720 | This work |
| YB779 | NA1000 pstC::miniTn5lacZ1101 | This work |
| YB1387 | NA1000 pstA::miniTn5lacZ1104 | This work |
| YB1389 | NA1000 pstA::miniTn5lacZ1102 | This work |
| YB778 | NA1000 pstA::miniTn5lacZ1103 | This work |
| YB783 | NA1000 phoBΩ12 pstA::miniTn5lacZ1103, transduction of Kanr from YB778 into YB720 | This work |
| YB777 | NA1000 pstB::miniTn5lacZ1105 | This work |
| YB784 | NA1000 phoBΩ12 pstB::miniTn5lacZ1105, transduction of Kanr from YB777 into YB720 | This work |
| YB1684 | NA1000 pstB::pBGST18pstB5 (pstB::np) | This work |
| YB1686 | NA1000 phoBΩ12 pstB::pBGST18pstB5 (pstB::np), integration of pBGST18pstB5 into YB720 | This work |
| Plasmids | ||
| pJM290 | 6.5-kb EcoRI fragment containing the C. crescentus pstB, phoU, and phoB genes cloned into pSKII− | This work |
| pXXphoUB19 | 1.0-kb XhoI fragment from pJM290 containing the C. crescentus phoB gene cloned into pKSII+ in the orientation of lacZ | This work |
| pXXphoUB48 | 1.0-kb XhoI fragment from pJM290 containing the C. crescentus phoB gene cloned into pKSII+ in the opposite orientation of lacZ | This work |
| pNPTS138/phoB21 | 1-kb XhoI fragment containing phoB cloned into the SalI site of plasmid pNPTS138 | This work |
| pphoBΩ12 | 2-kb Ω fragment of pHP45Ω cloned into the SmaI site of pNPTS138/phoB21 | This work |
| pGL10phoB | 1.0-kb XhoI fragment from pJM290 containing the C. crescentus phoB gene cloned into the SalI site of plasmid pGL10 | This work |
| pBGST18pstB5 | 400-bp amplified internal fragment of pstB cloned into pBGST18; used to disrupt pstB | This work |
| pGL10 | oriT Kanr | D. Helinski |
| pNPTS138 | Litmus 38-derived vector; oriT sacB Kanr | Alley, unpublished results |
| pHP45Ω | pBR322 derivative, Ampr, contains a 2-kb Ω Specr element | 35 |
| pBGST18 | A derivative of pBGS18 containing an RK2 oriT fragment | 45; Alley, unpublished results |
| pSKII+ | Phagemid, Ampr ColE1 ori f1 ori | 2 |
| pSKII− | Phagemid, Ampr ColE1 ori f1 ori | 2 |