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. 2022 Aug 17;10:901320. doi: 10.3389/fcell.2022.901320

FIGURE 2.

FIGURE 2

Depletion of most 19S RP subunits severely decreases proteolytic activity. (A) Representative images of germ line from Ub(G76V)::GFP::H2B animals treated with the indicated RNAi. Representative images of normal germline proteolytic activity [control(RNAi) and rpt-6(RNAi)], severe dysfunction of proteolytic activity [rpn-11(RNAi)], and moderate dysfunction of proteolytic activity [rpn-9(RNAi)]. A gonad arm is outlined with white dashed lines. (B) Average fluorescence intensity of Ub(G76V)::GFP::H2B germ lines treated with either RNAi against a control (n = 122) or any of the various 19 subunits of the 19S RP (n = 10–52). Fluorescence intensity (a. u) was measured in the region outlined with the white dashed lines as indicated in (A). All images taken at the same laser intensity and PMT gain, and then the same post-image modifications made to each image. **** represents p-values < 0.0001 compared to control(RNAi) condition. Error bars represent SEM. Scale bar, 50 µm.