FIGURE 5.

XND-1 turnover is affected by knockdown of a subset of 19S RP non-ATPase subunits. Representative images showing defects in XND-1 turnover after depletion of a specific group of non-ATPase proteasome subunits. Anti-XND-1 (magenta); DAPI stained DNA (cyan). (A) Vector control. (B) rpn-3(RNAi), and (C) rpn-6.1(RNAi) are examples of two subunits whose knockdown causes persistence of high levels of nucleoplasmic XND-1 in late pachytene nuclei. (D) dss-1(RNAi) is representative of the class of subunits who depletion does not affect XND-1. Scale bar, 50 µm.