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. 2022 Aug 17;10:901320. doi: 10.3389/fcell.2022.901320

FIGURE 6.

FIGURE 6

WEE-1.3 function and localization are altered by depletion of specific proteasome subunits. (A) Average 24 h brood and WEE-1.3 nuclear localization status of hermaphrodites treated with either control(RNAi), wee-1.3(RNAi), cdk-1(RNAi) individually or co-depleted with WEE-1.3, or 19S RP subunits co-depleted with WEE-1.3 via RNAi (all co-depletions with WEE-1.3 are bolded). All co-depletion conditions were compared to WEE-1.3 co-depleted with the control RNAi condition. * represents p values < 0.001, Y (yes) or N (No) represents whether or not aberrant nuclear localization of WEE-1.3 occur when control or proteasome subunits depleted individually. ND indicated not determined. (B) Live imaging of gonads from strain WDC2 wee-1.3[ana2(gfp::wee-1.3)] treated with either control(RNAi), rpn-6.1(RNAi) or dss-1(RNAi). All images were taken at the same laser intensity and PMT gain. Scale bar, 100 µm.