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. 2022 Aug 17;10:901320. doi: 10.3389/fcell.2022.901320

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Copyright © 2022 Fernando, Quesada-Candela, Murray, Ugoaru, Yanowitz and Allen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The two RPN-6.1 isoforms exhibit different spatial localization. Live imaging of hermaphrodites expressing endogenously GFP-tagged (A) RPN-9 and (B) RPN-6. Strains are WDC5 rpn-9[ana5 (gfp::rpn-9)] and WDC3 rpn-6.1a [ana3(gfp::rpn-6.1a)]. (C) Immunofluorescence image of rpn-6.1[ana12(rpn-6.1::ollas)] strain dissected gonad co-stained with anti-OLLAS (red), anti-pH3 (green, condensed chromatin) and DAPI for DNA (blue). Anti-pH3 was used solely to confirm presence of maturing oocytes. Bright nuclear and relatively dim cytoplasmic RPN-6.1b expression shown throughout germ line. Scale bar, 50 µm.