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. 2022 Aug 17;10:901320. doi: 10.3389/fcell.2022.901320

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Copyright © 2022 Fernando, Quesada-Candela, Murray, Ugoaru, Yanowitz and Allen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RPN-6.1 and RPN-7 are required for the nuclear localization of RPN-8 and RPN-9. (A) Live imaging of hermaphrodite oocytes from endogenously GFP tagged strains rpn-7[ana1(gfp::rpn7)], rpn-8[ana4(gfp::rpn-8)], rpn-9[ana5(gfp::rpn-9)] and rpn-12[ana6(gfp::rpn-12)] treated with either control(RNAi), rpn-6.1(RNAi) or rpn-7(RNAi) (n = 15–42). Scale bar represents 25 µm. (B) Model for role of RPN-6.1 and RPN-7 in nuclear localization of 19S RP lid combining existing information on eukaryotic proteasome assembly model (Budenholzer et al., 2017; Bai et al., 2019).