TABLE 2.
Summary of the germline phenotypes associated with RNAi-depletion of the various 19S RP subunits.
| Gene RNAi | Emb lethal a | Effect on brood b | Effect proteolytic activity c | MZ defects d | PCs/Premature polarization e | Defective XND-1 turnover f | Aberrant nuclear WEE-1.3 g | Suppress wee-1.3(RNAi) infertility h |
|---|---|---|---|---|---|---|---|---|
| rpn-1 | 1 | 1 | + | + | +/+ | + | + | no |
| rpn-2 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-3 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-5 | 1 | 2 | + | + | +/+ | + | + | + |
| rpn-6.1 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-7 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-8 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-9 | 3 | 3 | + | + | no | no | + | + |
| rpn-10 | 5 | 2 | no | none | no | no | no | no |
| rpn-11 | 1 | 1 | + | + | +/+ | + | + | + |
| rpn-12 | 4 | 2 | + | none | no | no | + | no |
| rpn-13 | 5 | 3 | no | none | no | no | no | no |
| dss-1 | 4 | 3 | no | none | no | no | no | no |
| rpt-1 | 1 | 1 | + | + | +/n.d | + | + | no |
| rpt-2 | 1 | 1 | + | + | +/+ | + | + | + |
| rpt-3 | 1 | 1 | + | + | +/+ | + | + | no |
| rpt-4 | 2 | 1 | + | + | +/+ | + | + | no |
| rpt-5 | 1 | 1 | + | + | +/+ | + | + | no |
| rpt-6 | 3 | 2 | no | + | no | no | no | no |
| Control | 5 | 4 | no | none | no | no | no | no |
| Bortezomib | 1 | 1 | + | + | +/no | no | no | no |
1 < 5% hatching; 2 = 5%–39%; 3 = 40%–74%; 4 = 75%–97%; 5 = no defect.
Average 24 h brood: 1 < 10 progeny; 2 = 11–75; 3 = 76–150; 4 > 150.
No = does not result in statistically significant difference in expression of germline proteolytic reporter. + results in a statistically significant increase in expression of the germline proteolytic reporter.
(+) Cell cycle defects in the adult germ line after knocking down RP subunits by RNAi. None = no cell cycle defects in the adult germ line after knocking down RP subunits by RNAi.
(+) SC polycomplexes and premature polarization of SYP-1 after knocking down RP subunits by RNAi. No = no SC polycomplexes and premature polarization of SYP-1 after knocking down RP subunits by RNAi.
(+) Defective XND-1 turnover in late pachytene after knocking down RP subunits by RNAi. No = normal XND-1 turnover in late pachytene after knocking down RP subunits by RNAi.
No = no WEE-1.3 nuclear localization. + results in aberrant WEE-1.3 nuclear localization.
No = does not result in a statistically significant suppression of wee-1.3(RNAi) infertility. + results in a statistically significant suppression of wee-1.3(RNAi) infertility.