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. 2022 Aug 17;13:989242. doi: 10.3389/fmicb.2022.989242

FIGURE 5.

FIGURE 5

Binding of the recombinant VP8* proteins from the RVA strains 14R103 (G5P[6]), 13R054 (G5P[7]), 12R010 (G4P[13]), 12R046 (G9P[23]) and 12R022 (G2P[27]) to synthetic oligosaccharides. Polyacrylamide (PAA)-biotin conjugates of LewisX, β-lactose, cellobiose and L-Rha [negative control [NC)] were used in the binding assay. The GST-VP8* fusion proteins from RVAs 14R103 (G5P[6]), 13R054 (G5P[7]), 12R010 (G4P[13]), 12R046 (G9P[23]), and 12R022 (G2P[27]) were used in the binding assay to confirm the binding specificity. GST-tagged recombinant proteins and the GST tag alone were tested. Error bars in the panel represent the means ± the SD from triplicate experiments. ***, P < 0.001. *, P < 0.05. ns, P > 0.05.