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. 2022 Aug 17;13:989242. doi: 10.3389/fmicb.2022.989242

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Copyright © 2022 Ren, Saleem, Haes, Xie, Theuns and Nauwynck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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β-lactose pretreatment decreases the replication of rotavirus in porcine enterocytes. After isolation, the ileum enterocytes were incubated with lactose pretreated RVAs for 12 h. (A) The viability of clusters during incubation was checked with EMA. The percentage of infected clusters (B-13R054 G5P[7] and C-12R046 G9P[23]) and the percentage of infected cells in a single cluster (D-13R054 G5P[7] and E-12R046 G9P[23]) were determined. (A–C) The error bars in the figures represent the means ± the SD from triplicate experiments. (D,E) The error bars in the figures represent the min to max. *P < 0.05. ns, P > 0.05.