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. 2018 Mar 13;22(2):99–105. doi: 10.1016/j.bjid.2018.02.006

Fig. 2.

Fig. 2

Sensitivity of multiplex PCR. Electrophoresis gel showing the amplification of different dilutions of CoNS and S. aureus. Lane 1: 100–2000 bp molecular weight marker; 2: mecA-positive S. aureus ATCC; 3: S. epidermidis ATCC; 4: CoNS 10−7 dilution (10 CFU/mL); 5: 10−6 (102 CFU/mL); 6: 10−5 (103 CFU/mL); 7: 10−4 (104 CFU/mL); 8: 10−3 (105 CFU/mL); 9: 10−2 (106 CFU/mL); 10: 10−1 (107 CFU/mL), 11: S. aureus 10−7 dilution (10 CFU/mL); 12: 10−6 (102 CFU/mL); 13: 10−5 (103 CFU/mL); 14: 10−4 (104 CFU/mL); 15: 10−3 (105 CFU/mL); 16: 10−2 (106 CFU/mL); 17: 10−1 (107 CFU/mL); 18: 100–2000 bp molecular weight marker.