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. 2022 Aug 17;10:938520. doi: 10.3389/fbioe.2022.938520

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Copyright © 2022 Lan, Yao, Li, Liu, Wang, Wang and Qi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of the protection of different concentrations of tocopherol in H₂O₂-stimulated BMSCs. (A) Cell viability of H₂O₂-stimulated BMSCs after tocopherol (1–200 μM) treatment was detected by the CCK-8 assay. (B) Intracellular ROS measurement was measured by microscopy (scale bar = 100 μM). (C) Intracellular ROS measurement was measured by flow cytometry. (D–G) RT‐PCR analysis measured the ROS level of H₂O₂-stimulated BMSCs (*p < 0.05; **p < 0.01 vs. the H₂O₂ group).