FIGURE 5.

Exploration of the anti-oxidative stress mechanism of tocopherol. (A) Signaling pathways associated to oxidative stress were determined by Western blot. (B) Adding the ferroptosis inhibitor ferrostatin-1 (5 μM), PI3K/AKT/mTOR-related protein levels were determined by Western blot. (C) After treated with 2 μM 740-YP (a PI3K agonist), GPX4, xCT, and ACSL4 protein levels were determined by Western blot. (D–G) Nrf2, Gpx4, Ptgs2, and Ncoa4 mRNA levels were determined by RT-PCR. (*p < 0.05; **p < 0.01 vs. H₂O₂ group)