Figure S1.
BCMA signaling is essential for the growth and survival of MM. (A) BCMA mRNA expression in a panel of 18 tumor cell lines queried through Oncomine. BCMA expression is significantly elevated in myeloma cell line; P = 5.45 × 10−31. (B) Western blotting analysis confirming knockdown of BCMA in MM cell line by dox-inducible shRNA. (C) Tumor growth kinetics in MM1.R MM cells transfected with inducible dox shBCMA, mice were given drinking water with or without dox (5 mg/ml); P = 0.007. (D) Quantitative analysis of Ki67-positive cells in the harvested tumors of dox-inducible shSCRM and shBCMA, represented as the average number of positive nuclei per image field; P = 0.0055. (E) Quantitative analysis of TUNEL-positive cells in the harvested tumors of dox-inducible shSCRM and shBCMA, represented as the average number of positive nuclei per image field; P = 0.0026. Statistical analysis was conducted using one-way ANOVA for comparing between treatment groups and repeated ANOVA for changes occurring over time. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Source data are available for this figure: SourceData FS1.