Figure S2.

BCMA is a critical regulator of protein translation machinery. (A) Loss of BCMA-induced changes in downstream targets associated with protein translation analyzed through RPPA. (B) Spearman correlation analysis of ribosome profiling samples showing consistent reads across all samples with the exception of RFP3. (C) Spearman correlation analysis after RFP3 was removed from the dataset. (D) Distribution plot of changes in mRNA expression associated with genetic knockdown of BCMA. (E) Volcano plot analysis identifying significant changes of downstream targets within total RNA transcript associated with BCMA knockdown. Color-coded dots show statistical significance associated with log₁₀Fc, P value, or both. FC, fold-change. (F) Volcano plot analysis identifying significant changes of downstream targets within ribosome-bound RNA associated with BCMA knockdown. Color-coded dots show statistical significance associated with identified targets categorized into log₁₀Fc, P value, or both. (G) Representative heatmap of RPPA analysis showing subset of the significant changes in protein expression landscape associated with BCMA signaling axis. (H) Graphic illustration of large ribosome subunits (top) and small ribosome subunits (bottom). Each subunit shows changes in translation abundance upon BCMA loss. Decreased expression (green), no change (gray), or increased expression (red).