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. 2022 Aug 17;13:971846. doi: 10.3389/fpls.2022.971846

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Copyright © 2022 Muñoz-Avila, Prieto, Sánchez-Sevilla, Amaya and Castillejo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression analysis in 35S:FaSOC1 lines (SOE). (A) FaSOC1 overexpression in leaves (green, left panel) and crown (purple, right panel) of three independent SOE lines. (B) FaCO, FaTEM and FaFT1 expression in leaves of SOE lines. (C) Flowering time gene expression in crowns from SOE lines. All samples were collected under natural SD (9 h52 min/14 h17 min day/night; average maximum and minimum temperature from previous 4 weeks: 21.1/10.6°C) conditions at ZT3. Graphs show the average of normalized values from three biological replicates ± SEM. In each bar, dots represent the normalized value of each biological replicate. *p < 0.05, **p < 0.01, ***p < 0.001.