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. 2022 Aug 17;13:930804. doi: 10.3389/fphys.2022.930804

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Copyright © 2022 Pisciotta, Lunghi, Bertani, Di Tinco, Bertoni, Orlandi, Biscarini, Bianchi and Carnevale.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of neurogenic differentiation of hDPSCs on PEDOT: PSS films. The expression of the neuronal markers $β$ —Tubulin-III (green) and MAP-2 (red) in hDPSCs cultured in neurogenic medium for 7 days on both types of PEDOT:PSS films are shown by representative immunofluorescence images. Nuclei were counterstained with DAPI. hDPSCs differentiated on plastic culture plates were used as controls. Scale bar: 20 μm (A). Real-time PCR analysis showing fold increase of mRNA levels of $β$ —Tubulin-III, MAP-2, NeuN and Synaptophysin (SYP) in hDPSCs after 3 weeks of neurogenic induction on ED-PEDOT films. Data represent mean ± SD of fold change obtained from three independent experiments. ^* p < 0.05, ^*** p < 0.001 vs. neurodiff hDPSCs (i.e., on plastic culture plates) (B).