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. 2022 Aug 17;13:920280. doi: 10.3389/fmicb.2022.920280

FIGURE 2.

FIGURE 2

Detection of the interaction between preS1 and NTCP by cell-surface display. (A) Detection of preS1-NTCP interaction by the cell-surface display. Wild type NTCP-mCherry was displayed on the cells, and preS1-GFP was used as the bait protein. The interaction was abolished in both the preS1 mutants and the NTCP mutants. (B) The preS1 peptides were displayed on the cell surface, and NTCP-GFP on the membrane was used as the bait protein. The mutated NTCP (NTCP d157-165 or 267S/F) lost its affinity for the preS1 peptide, leading to no or low green fluorescence on the Cherry-FasL-preS1-48 cells. The affinity of preS1-48 for NTCP is higher than that of preS1-21, resulting in more green fluorescence on the Cherry-FasL-preS1. (C). Increasing the amount of NTCP-GFP (from left to right) led to increased green fluorescence detected on the Cherry-preS1-21 cells. (D). Competitive assays. Myr-preS1-21s or control peptide was used to compete with preS1-21 on the cell surface.