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. 2022 Aug 17;13:920280. doi: 10.3389/fmicb.2022.920280

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Copyright © 2022 Wang, Yang, Guo, Wang, Zhang, Sun, Li, Gan, Long, Liu, Fan, Huang and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and screening of the random peptide library. (A) Sanger sequencing results of the library (PT4-21 + 7). (B) Deep sequencing results of the initial or enriched sequence. The number of unique sequences (x-axis) and the proportion of each sequence (y-axis) are shown. (C) The 10 sequences with the highest enrichment fold and proportion after 7 rounds of screening. (D) Evaluation of the 10 sequences selected by flow cytometry. The peptides fused with GFP were expressed by HEK293 cells and captured by the cells displaying NTCP-mCherry. Data are shown as means ± SD. Statistical significance was determined using the non-parametric Mann–Whitney U-test (*p < 0.05, **p < 0.01).