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. 2022 Aug 17;13:920280. doi: 10.3389/fmicb.2022.920280

FIGURE 4.

FIGURE 4

Effects of combining pep3 with different genotypes of preS1-21. (A) Plasmids expressing pep3-GFP fused with preS1-21 from genotype A to F and the consensus sequence of A to F were constructed for the evaluation. (B) Evaluation of the binding capability for NTCP of pep3 combined with preS1-21 from different genotypes. preS1-21-pep3-F and preS1-21-pep3-con showed the highest binding capability whereas preS1-21-pep3-E the lowest. Western blot was used to check the expression levels of different proteins. (C) Evaluation of preS1-21-pep3-con’s ability to bind NTCP. PreS1-21-pep3-con showed a higher binding capacity than preS1-21-D, and preS1-21-pep3-con demonstrated a higher binding capacity than preS1-21-pep3-D. Notably, compared to preS1-21-D and preS1-21-con, both preS1-21-pep3-D and preS1-21-pep3-con showed a much higher binding capability. (D) Evaluation of the binding ability by confocal microscopy. preS1-21-pep3-con and preS1-21-pep3-D displayed an apparently higher binding to the cell membrane (green fluorescence) than preS1-21-con and preS1-21-D, while the negative control (GFP-FRB) had only scatter background.