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. 2022 Oct 13;185(21):4008–4022.e14. doi: 10.1016/j.cell.2022.08.024
REAGENT or RESOURCE SOURCE IDENTIFIER
Flow cytometry staining reagents

PE anti-DYKDDDDK Tag Antibody Biolegend Cat# 637309;
anti-human IgG-AlexaFluor647 Jackson Immunoresearch 109-605-098;
biotinylated human ACE2 Acro Cat# AC2-H82E6
streptavidin-AlexaFluor 647 Biolegend Cat# 405237

Deposited data

Raw sequencing data Starr et al. 2020 NCBI SRA: BioProject PRJNA639956
Raw and processed sequencing data This study

Oligonucleotides

Degenerate Ultramers and oPools for RBD library construction IDT https://github.com/LSSI-ETH

Recombinant DNA

pYD1-RBD(wt) This study https://github.com/LSSI-ETH; derived from RRID:Addgene_73447

Cell lines

EBY100 ATCC Cat# MYA-4941

Software and algorithms

bbduk Joint Genome Institute https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbduk-guide/
custom scripts for curation, analysis, and visualization This study https://github.com/LSSI-ETH