Fig. 2.

a, Representative western blot of p-MLKL protein levels in pancreas from wild-type (WT) and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein injection. β-tubulin was used as loading control. b, Plasma levels of cell free DNA (cfDNA) and c, plasma levels of MLKL in wild-type (WT) and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein injection. d, Representative histological images of hematoxylin-eosin staining and histological score for necrosis of pancreas from wild-type (WT) and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein injection (Scale bar, 100 μm). e, Representative western blot of PGC-1α, f, SRX and hyperoxidized forms of PRX1, PRX2 and PRX3 protein levels in pancreas from wild-type (WT) and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein injection. β-tubulin was used as loading control. g, mRNA relative expression of Prx3 vs. Tbp in pancreas from wild-type (WT) mice and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein. h, Representative western blot of PRX3 protein levels in pancreas from wild-type (WT) and p53 knockout (KO) mice under basal conditions (sham) and with acute pancreatitis at 24 h after the first cerulein injection. β-tubulin was used as loading control. Data are presented as mean ± SD, **P < 0.005, *P < 0.05 (two-way ANOVA, followed by Tukey's multiple comparisons test).