Fig. 3.

a, mRNA relative expression of Srxn1 vs. Tbp in pancreas from control mice at time 0 h and from mice with acute pancreatitis 7 h and 24 h after the first cerulein injection. Data are presented as mean ± SD, **P < 0.005 (one-way ANOVA, followed by Tukey's multiple comparisons test). b, Representative western blot and densitometry quantification showing SRX protein levels in pancreas from control mice at 0 h and from mice with acute pancreatitis at 7 h and 24 h after the first cerulein injection. β-tubulin was used as loading control. c, mRNA relative expression of Srxn1 vs. Tbp in pancreas from control mice at 0 (n = 6), 1 (n = 3), 3 (n = 3), 5 (n = 3), and 7 h (n = 8) after the first cerulein injection. d, Representative western blot and densitometry quantification of SRX protein levels in pancreas from control mice at 0, 1, 3, 5, and 7 h after the first cerulein injection (n = 4 mice per group). β-tubulin was used as loading control. e, Representative western blot and densitometry quantification showing hyperoxidized forms of PRX1, PRX2 and PRX3 in pancreas from control mice at 0, 1, 3, 5, and 7 h after the first cerulein injection (n = 4 mice per group). β-tubulin was used as loading control. f, Levels of H₂O₂ in pancreas from control mice at 0 (n = 4), 1 (n = 3), 3 (n = 3), 5 (n = 3), and 7 h (n = 8) after the first cerulein injection. g, Representative western blot of SRX in cytosolic and mitochondrial fraction of pancreas from sham mice and at 7 h after the first injection of cerulein (n = 4 mice per group). TOM20 was used as loading control for mitochondrial fraction. β-tubulin was used as loading control for cytosolic fraction. Data are presented in panels c-f as mean ± SD, **P < 0.005, *P < 0.05 (each time point vs. control mice at time 0 by one-way ANOVA, followed by Bonferroni's multiple comparisons test).