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. Author manuscript; available in PMC: 2022 Aug 31.
Published in final edited form as: Exp Eye Res. 2022 May 23;220:109125. doi: 10.1016/j.exer.2022.109125

Fig. 1. Expression of CGRP and its receptor.

Fig. 1.

(A–C) Representative images of CGRP (red) and β-tubulin III (nerve marker, green) double immunofluorescence showing the distribution of CGRP in the trigeminal ganglion (TG, A), cornea (B) and cultured TG neurons (C); arrows showing representative double-positive neurons/nerves. (D) CGRP positivity was calculated as percentage of CGRP-positive versus total β-tubulin III-positive neurons or nerves (n = 3). (E) CGRP mRNA expression levels in TG and cornea, (n = 3). (F) Corneal intrastromal suture was placed in the mouse cornea to induce corneal neovascularization (CNV) for 1 week. The expression of CGRP in TG and cornea and the expression of CGRP receptor CLR and RAMP1 in the cornea were determined using RT-PCR (n = 6) and expressed as number of copies in log scale per 106 GAPDH (internal control). *p < 0.05 unpaired t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)