TABLE 1.
Bacterial strains used in this work
| Strain | Relevant genotype or characteristicsa | Source or reference |
|---|---|---|
| E. coli | ||
| BL21(DE3) | lon ompT hsdS, lysogenized with DE3, a λ phage carrying the T7 RNA polymerase gene under the control of the lacUV5 promoter; host strain for overexpression of genes cloned in vector pET3d | 65 |
| CC118λpir | Rifr, araD139 Δ(ara-leu) araD ΔlacX74 galE galK phoAΔ20 thi-1 rpsE rpoB argE recA1, lysogenized with λpir; host strain to propagate plasmids with a R6K origin of replication | 22 |
| DH5α | F−supE44 (φ80dlacZΔM15) Δlac(lacZYA-argF)U169 hsdR17 recA1 endA1 gyrA96 (Nalr) thi-1 relA deoR; host strain in routine cloning experiments | Gibco BRL, Life Technologies |
| HB101 | Smr, supE44 hsdS20 recA13 leuB6 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | 7 |
| S17-1λpir | Smr Tpr, supE44 endA1 recA thi pro hsdR RP4-2-Tc::Mu-Km::Tn7, RP4 derivative integrated in the chromosome, lysogenized with λpir, donor strain in biparental matings to mobilize RP4 oriT containing plasmids lacking res sites | 14 |
| P. azelaica | ||
| HBP1 | 2-HBP+, 2-PP−, wild-type | 30 |
| HBP1 Prp | 2-HBP+, 2-PP+, spontaneous mutant obtained from strain HBP1 | 32 |
| HBP104 | Kmr, 2-HBP+, 2-PP−, contains mini-Tn5 (PhbpC− luxAB res-npt-res) | This work |
| HBP104 Prp | Kmr, 2-HBP+, 2-PP+, contains mini-Tn5 (PhbpC− luxAB res-npt-res) | This work |
| HBP121 | Smr Tcr, 2-HBP−, 2-PP−, plasmid pHYBP121 integrated in hbpR | This work |
| HBP127 | Kmr Smr Tcr, 2-HBP+, 2-PP−, plasmid pHYBP121 integrated in hbpR; contains mini-Tn5 (hbpR res-npt-res) | This work |
| HBP129 | Kmr Smr Tcr, 2-HBP+, 2-PP+, plasmid pHYBP121 integrated in hbpR; contains mini-Tn5 (hbpR-T613C res-npt-res) | This work |
| HBP104121 | Kmr Smr Tcr, 2-HBP−, 2-PP−, plasmid pHYBP121 integrated in hbpR; contains mini-Tn5 (PhbpC-luxAB res-npt-res) | This work |
a
2-HBP+/−, ability/inability to grow on 2-hydroxybiphenyl; 2-PP+/−, ability/inability to grow on 2-propylphenol; hbpR-T613C, hbpR gene isolated from strain HBP1 Prp; res-npt-res, element with the kanamycin resistance gene of Tn5 (npt) flanked by tandem multimer resolution sequences (res) of RP4 (33).