Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 30;12:140. doi: 10.1186/s13578-022-00876-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Motoneuron innervation is required for specialization of synaptic nucleus. A Whole-mount staining of TA muscles in ChATBCA-eGFP mice (2-month-old) after denervation for 3, 7, 16, or 30 days. BTX (red), DAPI (blue), ChAT-eGFP (green). Note that synaptic nucleus were gradually dispersed. D, denervation. B Quantification in A. n = 19 NMJs in Sham, n = 21 NMJs in D3, n = 21 NMJs in D7, n = 30 AChR cluster area in D16, n = 38 AChR cluster area in D30. Images were from three mice. One-way ANOVA with Tukey’s post hoc test for multiple comparisons F (4,127) = 26.46. C DAPI staining in SR/NSR in sham and denervated muscles. DAPI (blue), R-BTX (red). D Quantification in C. Sham, n = 63 nuclei in NSR and n = 63 nuclei in SR; D30, n = 74 nuclei in NSR and n = 49 nuclei in AChR cluster area. Images were from three mice. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons. SR&NSR: F (1,245) = 183.3; Innervation & Denervation: F (1,245) = 22.01. E Whole-mount staining of TA muscles in 4-month-old SOD1^G93A mice and littermate controls. BTX (red), DAPI (blue), SV2 (green). SV2 (Synaptic vesicle glycoprotein 2) is a synaptic protein to indicate presynapse at nerve terminals. F Quantification of synaptic nucleus number in SOD1^G93A mice at indicated ages. Control mice: n = 25 NMJs (or images) in P60, n = 20 in P90, n = 21 in P120, n = 13 in P150. SOD1^G93A mice: n = 25 NMJs (or images) in P60, n = 25 in P90, n = 26 in P120, n = 24 in P150. Images were from three littermate mice. Two-way ANOVA with Sidak’s post hoc test for multiple comparisons. Age: F (3,171) = 14.2; genotype: F (1,171) = 64.38. G Quantification of synaptic nucleus number in SOD1^G93A mice at indicated ages. Control mice: n = 130 nuclei in NSR and 120 in SR in P60, n = 102 nuclei in NSR and 94 in SR in P90, n = 72 nuclei in NSR and 55 in SR in P120, n = 70 nuclei in NSR and 60 in SR in P150. SOD1^G93A mice: n = 96 nuclei in NSR and 89 in SR in P60, n = 103 nuclei in NSR and 70 in SR in P90, n = 109 nuclei in NSR and 86 in SR in P120, n = 128 nuclei in NSR and 78 in SR in P150. Images were from three littermate mice. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons. Age: F (3,1446) = 9.626; SR&NSR: F (3,1446) = 313.1. Unless otherwise specified, three independent experiments were performed; the mean ± SEM is shown. ***p < 0.001, ns, no significance