TABLE 1.
Escherichia coli K-12 strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source and/or reference |
|---|---|---|
| E. coli K-12 strains | ||
| AB1157 | thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 tsx-33 glnV44(AS) galK2(Oc) hisG4(Oc) rfbD1 mgl-51 rpoS396(Am) rpsL31(Strr) kdgK51 xylA5 mtl-1 argE3(Oc) thi-1 | E. A. Adelberg |
| AB1874 | F-42 (F-lac)/lac-19 | E. A. Adelberg |
| AM207 | As AB1157 but recR252::mTn10 | R. G. Lloyd |
| AM547 | As AB1157 but ΔruvAC65 | R. G. Lloyd |
| DE407 | lexA3 mal::Tn9 sulA211 rpsL31 Δ(lac-gpt)5 mtl-1? thi-1? | D. Ennis (4) |
| CS81 | As AB1157 but ruvB52 eda-51::Tn10 | R. G. Lloyd |
| GM698 | As KL14 but dam-13::Tn9 | Lab stock |
| GM2807 | As KL14 but dam-16::Kan | Lab stock |
| GM2835 | As KL14 but aroK17::Cam | Lab stock |
| GM7362 | As DE407 but mutL218::Tn10 | This work |
| GM7363 | As DE407 but mutS215::Tn10 | This work |
| GS1481 | As AB1157 but ΔruvC64::Kan | R. G. Lloyd |
| JC10990 | AS AB1157 but recF332::Tn3 | M. Volkert |
| JC13028 | As AB1157 but recJ147(Ts) | S. Lovett |
| KD2250 | As AB1157 but recQ1803::Tn3 | H. Nakayama |
| KL14 | Hfr relA1 spoT1 thi-1 | K. B. Low |
| KM353 | As AB1157 but recD1901::Tn10 | K. M. Murphy |
| N2057 | As AB1157 but ruvA60::Tn10 | R. G. Lloyd |
| N2446 | As AB1157 but recJ284::Tn10 | R. G. Lloyd |
| N3793 | As AB1157 but ΔrecG263::Kan | R. G. Lloyd |
| Plasmids | ||
| precA | A pBR322 derivative with the recA gene under pTac control, Ampr | K. Knight (10) |
| precAP67W | A P67W mutant derivative of precA causing coprotease constitutivity | K. Knight (10) |
| pGB2 | Cloning vector derived from pSC101, Spcr | B. Michel (26) |
| pGB2ruvAB | pGB2 with cloned ruvAB genes | B. Michel (26) |