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. Author manuscript; available in PMC: 2023 Jan 5.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Dec 23;33(1):181–188. doi: 10.1021/jasms.1c00318

Figure 3.

Figure 3.

Following affinity selection using MagMASS, positive ion electrospray LC-MS was used to detect ligands that bound to the SARS-CoV-2 spike S1 protein. Selective binding of a ligand to the native S1 protein (solid line) is indicated by peak enhancement relative to denatured S1 protein (dashed line). (A) The positive control SBP-1 peptide bound to the native but not to denatured S1 protein (selected reaction monitoring of the [M+4H]4+ precursor). (B) and (C) High-resolution computer-reconstructed mass chromatograms showing two hits detected during the screening of a licorice root extract (Glycyrrhiza inflata). Hit 1 was identified as licochalcone A by comparison with authentic standard (see Figure 4).