FIG 2.
Filamentation assays in YPD and RPMI with serum media. (A) Cells of genotype EFG1/EFG1 or efg1Δ/Δ and otherwise wild type or otherwise homozygous for PRBT5-UME6, PTDH3-UME6, PRBT5-HGC1, or PTDH3-HGC1 as indicated were assayed for filamentation under planktonic growth conditions. Strains were grown in YPD medium overnight at 30°C with shaking or transferred to RPMI with 10% serum at 37°C for 4 h after YPD overnight growth with shaking. Fixed cells were stained with calcofluor white for confocal microscopy. White scale bar, 20 μm. (B) EFG1/EFG1 and efg1Δ/Δ PRBT5-UME6/PRBT5-UME6 strains from the indicated clinical isolate backgrounds were assayed for filamentation as described for panel A. White scale bar, 20 μm.