FIG 3.
Effect of iron chelation on RBT5 promoter constructs. (A) Filamentation assays of efg1Δ/Δ, efg1Δ/Δ PRBT5-UME6/PRBT5-UME6, and efg1Δ/Δ PRBT5-HGC1/PRBT5-HGC1 strains were grown in YPD plus 0, 20, 50, 100, 200, 300, or 400 μM BPS at 37°C for 4 h with shaking. Fixed cells were stained with calcofluor white for confocal microscopy. The white scale bar (top left panel) represents 50 μm. (B) Cell body lengths were quantified with ImageJ, with a minimum of 100 cells for each BPS concentration. Values shown are means with SD. Pairs of means connected by a horizontal bar are significantly different (Tukey-Kramer test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).