FIG 5.

Expression levels of RBT5 promoter constructs. Strains were grown in RPMI with 10% serum at 37°C for 4 h with shaking. qRT-PCR analysis for RNA levels of UME6 or HGC1 under control of their native promoters, the RBT5 promoter, or the TDH3 promoter were measured and normalized to the level of ACT1 mRNA. Results from three independent experiments are shown. Values shown are means (+SD). Pairs of means connected by a horizontal bar are significantly different (Tukey-Kramer test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). (A) Strains were genotype efg1Δ/Δ and homozygous for the hybrid UME6 or HGC1 alleles indicated. (B) Strains were genotype EFG1/EFG1 and homozygous for the hybrid UME6 or HGC1 alleles indicated.