TABLE 2.
Primers
| Primer name | Sequencea |
|---|---|
| RBT5/F | cgattcgatactaacgcaatcactatttttaatgatcctacttctatcccgaaaaag |
| RBT5/R | aacgcgttgggagctctcccatatggtagtattagttattagtgatagttagtgaagaattag |
| OE backbone/F | atatgggagagctcccaac |
| OE backbone/R | tagtgattgcgttagtatcgaatc |
| Multipromoter UME6/F | aaaaaagaattcttcgtaatatctatatagatatcttcatttaattttcttggttgtttgatattactttgttgtactttATCAAGCTTGCCTCGTCCCC |
| Multipromoter HGC1/F | cccaaactatacttcccaataaaagatagaaactcgcttacaacaacacaatcctgaagattattaaatctctaattttcATCAAGCTTGCCTCGTCCCC |
| Multipromoter UME6/R | ttcaactttattgtatcttctccataaggcgaatttggtgctgaagaagttgaatcgggtgtaaccatatgggtaatcatCGTTGGGAGCTCTCCCAATG |
| Multipromoter HGC1/R | atgtttttgtatggatgttgttgttgttgtttttgttgtgaaattgattttggagttaatggtttagttatatttatcatCGTTGGGAGCTCTCCCAATG |
| CaCas9/for | Atctcattagatttggaacttgtgggtt |
| CaCas9/rev | Ttcgagcgtcccaaaaccttct |
| UME6p sgRNA/F | caagaaaattatcaaattctgttttagagctagaaatagcaagttaaa |
| UME6p SNR52/R | agaatttgataattttcttgcaaattaaaaatagtttacgcaagtc |
| HGC1p sgRNA/F | gtgtgtatagtgtagtatccgtgttttagagctagaaatagcaagttaaa |
| HGC1p SNR52/R | acggatactacactatacacaccaaattaaaaatagtttacgcaagtc |
| UME6 qRT/F | tccactttaccattatccaagtctactc |
| UME6 qRT/R | gtgttgataatgaatgaactaaatttgccc |
| HGC1 qRT/F | caccaccacaaatgcattctca |
| HGC1 qRT/R | atgaggtgcaggaagctgac |
| UME6p OE NAT1 TDH3/F | gggaaaaaagaattcttcgtaatatctatatagatatcttcatttaattttcttggttgtttgatattactttgttgtacATCAAGCTTGCCTCGTCCCC |
| UME6p OE TDH3 NAT1/R | ttcaactttattgtatcttctccataaggcgaatttggtgctgaagaagttgaatcgggtgtaaccatatgggtaatcatTGTTAATTAATTTGATTGTAAAGTTTGTTGATG |
| UME6p check up/F | gagagttttaatcaattagaaaccaacagagg |
| UME6p check int/R | cgaatgacaaagttaagtcaaaaattggacc |
| NAT1 check/R | tcaatggtggatcaactggaacttc |
| HGC1 OE/F | cccaaactatacttcccaataaaagatagaaactcgcttacaacaacacaatcctgaagattattaaatctctaattttcATCAAGCTTGCCTCGTCCCC |
| HGC1 OE/R | atgtttttgtatggatgttgttgttgttgtttttgttgtgaaattgattttggagttaatggtttagttatatttatcatTGTTAATTAATTTGATTGTAAAGTTTGTTGATG |
| HGC1 check/F | cttacattttagacgaccaacggatactaca |
| HGC1 check/R | cttcgattgaaggatcatttaaagaccattctaaa |
a
Nucleotides in bold uppercase letters are complementary to the pTH10 plasmid template.