Scheme 2. Microarray-patterned biochip design for duplex foodborne pathogen detection. (A) Preparation of E. coli–Salmonella (ES) biochip. Blue and green microarray spots indicate the locations for capture antibodies (anti-E. coli and anti-Salmonella, n = 10), respectively. The yellow spots show positive control (IgG-biotin, n = 28) and grey spots represent negative (1× PBS). (B) Preparation of Cy5-Ab complexes to amplify a foodborne detection signal. (C) ES-biochip for detecting foodborne pathogens. (D) Fluorescence image analysis.
