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High temperature increases the sensitivity of deletion strains to caspofungin. The KN99 and cft1Δ, erg4Δ, myo1Δ, and ysp2Δ strains were grown overnight in YPD and diluted to an OD650 of 1 before being spotted in 4-step serial dilutions on YPD agar plates containing 32 or 40 μg/mL caspofungin. The plates were incubated for 3 days at 37°C (A) or 39°C (B).
