TABLE 1.
Bacterial strains, plasmids, and bacteriophages used in this study
| Strain or plasmid | Relevant genotype and/or characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| χ711 | F−leu-4 proAB118 arg-35 λr T6r Strr | 7 |
| BW19851 | RP4-2::Mu-1kan::Tn7/creB510 hsdR17 endA1 zbf-5 uidA(ΔMluI)::pir(wt) recA1 thi | 32 |
| KLC2926 | W1485, rfaE::Tn10, Tetr Novs | This study |
| VW187 | E. coli O7:K1, prototrophic | 50 |
| VW194 | E. coli O6:K?, prototrophic | 52 |
| W1485 | E. coli K-12, wild type | E. coli Genetic Stock Center, Yale University |
| S. enterica | ||
| SL1027 | metA22 trpC2 fla-66 rpsL120 xyl-404 metE551 | 58 |
| SL1102 | Same as for SL1027, rfaE543 | 58 |
| R. eutrophus | ||
| HB3 | H16, aut::Tn5, deficient in autotrophic growth, Kmr | |
| H16 | Prototrophic strain | |
| Plasmids | ||
| pACYC184 | Cloning vector, Cmr | 9 |
| pBR325 | Cloning vector, Cmr Ampr Tetr | 3 |
| pCM199 | 1.4-kb amplicon containing the rfaE::Tn10 junction cloned into pUC19, Ampr | This study |
| pCM200 | 1.4-kb amplicon containing rfaE+ from S. enterica SL1027 cloned into pEX1, Ampr | This study |
| pCM202 | 1.4-kb amplicon containing rfaE543 from S. enterica SL1102 cloned into pEX1, Ampr | This study |
| pCM204 | 1.7-kb BamHI fragment containing the plac promoter and rfaE+ from pCM200 cloned into pME6000, Tetr | This study |
| pCM205 | 1.7-kb BamHI fragment containing the plac promoter and rfaE543 from pCM202 cloned into pME6000, Tetr | This study |
| pEX1 | Expression vector, Ampr | 37 |
| pHI3 | 1.9-kb HindIII-BglII fragment carrying part of the rfaE gene of H. influenzae 2019 cloned into pUC19, Ampr | 28 |
| pJB2 | 3-kb EcoRI fragment containing gmhA cloned into pMAV3, Cmr | 7 |
| pJK2252 | pGEM4 carrying a 5.5-kb BglII fragment harboring waaFCLKZ from E. coli K-12, Ampr | 22 |
| pMAV3 | Cloning vector, Cmr | 30 |
| pMAV44 | cat cassette inserted into the KpnI site of pTLS1, Cmr Ampr | This study |
| pMAV47 | 541-bp amplicon from pTLS1 cloned into pUC19, Ampr | This study |
| pMAV51 | 500-bp HindIII deletion from pTLS1, Ampr | This study |
| pMAV52 | 1.2-kb BamHI-HindIII fragment from pMAV51 cloned into pME6000, Tetr | This study |
| pMAV53 | 550-bp amplicon from pMAV47 cloned into pME6000, Tetr | This study |
| pMB10 | 1.9-kb BamHI fragment from pTLS1 cloned into pME6000, Tetr | This study |
| pME6000 | Cloning vector, Tetr | S. Heeb |
| pMGC2 | 12.5-kb BamHI fragment from E. coli VW194 cloned into pACYC184, Cmr | |
| pTLS1 | 1.4-kb amplicon containing the rfaE gene from pMGC2 cloned in pEX1, Ampr | This study |
| pUC19 | Cloning vector, Ampr | 59 |