FIG. 4.

Cell fractionation by sucrose density gradients of JM109DE3 cells containing pAA14 (A and C) and pAA16 (B and D), expressing WecA₃₆₇ and WecA₂₅₇, respectively. (A and B) Immunoblot analysis with MAb M2 of fractions collected from sucrose gradients. (C and D) Protein concentration (●) and NADH oxidase activity (□) profiles of the various fractions, as determined by measurement of A₃₄₀ (right vertical axis) and units of enzyme activity per milliliter (left vertical axis), respectively, are shown.